Figure 3.
Survival analysis of sulfasalazine (SSZ) treatment and antitumor effects for lung tumor nodules of SSZ alone, cisplatin alone, and their combinations in a murine lung metastasis model. A, Lung tumor nodules were generated by injecting 2 × 105 MBT‐2V cells into the tail veins of female C3H/HeN mice on day 0. The i.p. administration (2 days on/1 day off) of SSZ (500 mg/kg) or the vehicle control (PBS) was started on day 3 (n = 18 each group). Survival analysis was evaluated by Kaplan‐Meier curve between the SSZ treatment group and the control group. B, In the lung metastasis model, mice were classified into 4 groups: vehicle control, SSZ alone (500 mg/kg), cisplatin alone (2 mg/kg, every fifth day), and their combinations (n = 10 each group). Mice were killed on day 15, and the number of lung tumor nodules was counted macroscopically. *P < .01, **P < .001. C, Representative lungs extracted from mice treated with the vehicle control, SSZ alone, cisplatin alone, and their combination