Figure 6.

RNA sequencing reveals that the Hippo signaling pathway is enriched following stable silencing of Frizzled 2 (FZD2) in SNU449 cells. A, Volcano plot of differentially expressed genes (DEGs). Each dot represents a DEG. Significant DEGs (false discover rate [FDR] ≤ .001 and |Log2Ratio| ≥1) are indicated in red and nonsignificant DEGs in black. B, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of DEGs. C, Mammalian sterile 20‐like kinase (MST)1, MST2, large tumor suppressor kinase (LATS)1, phosphorylated Yes‐associated protein (p‐YAP), total YAP, and Tafazzin (TAZ) were detected by western blot analysis. D, Nuclear YAP was detected by western blot analysis. NC, negative control