Figure 1.

Suppressed expression of intraflagellar transport 20 (IFT20) impairs invasive migration of Ror2‐expressing colorectal cancer (CRC) cells. A, Expression levels of Ror2 and IFT20 in the CRC cell lines DLD1, HCT116, and SW480. Whole cell lysates from the respective CRC cells were analyzed by western blotting with Abs against the indicated proteins. B‐E, Decreased RNA (B,D) and protein (C,E) levels of IFT20 in HCT116 (B,C), but not SW480 cells (D,E), transfected with Ror2 siRNA. Cells were transfected with control (Ctrl), Ror2, or IFT20 siRNA and analyzed by real‐time PCR (B,D) and western blotting (C,E) to monitor amounts of the indicated RNAs and proteins, respectively. F‐I, HCT116 (F,G) and SW480 (H,I) cells were transfected with the indicated siRNAs and subjected to 2‐D invasion assay. Representative phase contrast images before (0 h) and after incubation for 18 h are shown (F,H). Scale bar, 500 μm. Invasion ratios of the respective cells after incubation for 18 h were determined (G,I). Data are expressed as mean ± SD (n = 3). *P < .05, t test