Figure 5.

Suppressed expression of intraflagellar transport 20 (IFT20) impairs polarized and continuous microtubule growth during collective invasion of colorectal cancer cells. DLD1 cells expressing EB1‐GFP were transfected with the indicated siRNAs and subjected to 2‐D invasion assay. After 16 h of incubation, movement of EB1‐GFP in leader cells was analyzed by time‐lapse fluorescence imaging [Link]and tracking programs. A, Circular histograms showing the percentages of EB1‐GFP comets whose moving direction was within each of 36 equal sectors (10°). 0° indicates the direction perpendicular to the leading edge of the original cell sheet. The analysis included 2300‐2500 comets from 15 cells per each condition. B,C, Quantification of pause frequency (B) and moving speed (C) of EB1‐GFP comets. We defined a pause as an absence of movement in either direction for at least 0.9 s. Data are presented as box‐and‐whisker plots. n = 300‐520 comets; ***P < .001, t test. si‐Ctrl, negative control siRNA