Figure 1.
Ring finger protein 115 (RNF115) is positively correlated with ubiquitin‐specific protease 9X (USP9X) expression in breast cancer cell lines and tumor samples. A, Immunoblotting analysis of USP9X and RNF115 in 31 pairs of breast cancer specimens (T) and matched adjacent noncancerous breast tissues (N). Western blots were quantified using ImageJ software (https://imagej.nih.gov/ij/). Protein levels of USP9X and RNF115 were normalized to those of internal control vinculin. Relative expression levels of USP9X and RNF115 (T/N) are shown under corresponding lanes in these western blots. B, The correlation between RNF115 and USP9X protein levels in these samples was analyzed using the χ2 test. R is Pearson's correlation coefficient. C, Immunoblotting analysis of USP9X and RNF115 proteins in 10 breast cancer cell lines and normal HMEC cell line. D, A correlation between the protein expression levels of RNF115 and USP9X in these cell lines was analyzed as described in B