Figure 2.

Ring finger protein 115 (RNF115) does not affect ubiquitin‐specific protease 9X (USP9X) expression. A, MCF‐7 and MDA‐MB‐231 cells were transfected with negative control siRNA (siNC) or 3 different siRNAs targeting RNF115 (siRNF115 #1‐3). After 48 h of transfection, cells were harvested and analyzed by immunoblotting with the indicated Abs. B,C, HEK293T cells were transfected with expression vector encoding empty vector pCDH or pCDH‐RNF115 with or without V5‐USP9X. After 48 h of transfection, total cellular lysates were subjected to immunoblotting with the indicated Abs