Figure 6.

mRNA expression analysis using the TOP RNA panel. A, mRNA expression clustering analysis for the 338 specimens was performed to investigate the clinical utility of the TOP RNA panel in the prediction of the primary organs of cancers of unknown primary sites or the histopathological identities of unclassified tumors. The 338 specimens analyzed by the TOP RNA panel V4 were classified into five large categories. Clusters 1, 2a, 3, 5a and 5b dominantly consisted of either sarcomas, lung cancers, gynecological cancers or colon adenocarcinoma. The tumor types are color‐coded in the upper label. CRAD, colorectal adenocarcinoma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; OV, ovarian cancer; SARC, sarcoma; SCLC, small cell lung cancer; SGC, seminoma and germ cell tumor; UCEC, uterine corpus endometrial carcinoma. B, Expression of the MET gene in a case of copy number amplification and of the ALK gene in fusion‐positive cases were quantified and compared with those of the other 80 cases of lung adenocarcinoma