Figure 5.
Expression analysis of the SlP4H3 in petiole AZs and leaf abscission rate after 48 h of ethylene treatment. (A) Relative expression of SlP4H3 transcript in three RNA lines and WT petiole AZs. The relative expression was calculated according to the comparative Ct method by using actin as internal standard. The asterisk indicates statistically significant differences. (B) The petiole abscission kinetics expressed as percentages of pedicel abscission were determined after deblading the leaf explants and treating them with approximately 15 μL L−1 of ethylene in the three SlP4H3 RNAi lines #1, #6, and #7 and the wild type. Three biological replicates were performed.