Table1.
Formulation variables used in preparation of BLF transferosomes
| No | Formulation | method | 3 LF concentration (mg/mL) | Surfactant |
4
S: L
ratio |
cholesterol | DOTAP | Size (nm) | 5 EE% | Zeta (mv) |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | S1 | TFH1 | 5 | Tween 80 | 1:10 | No | No | 237.0 | 24 | +0.1 |
| 2 | S2 | TFH | 5 | SDC | 1:10 | No | No | 2666.0 | 20 | +0.5 |
| 3 | S3 | TFH | 5 | Tween 80 | 1:10 | No | No | 160.0 | 30 | +1 |
| 4 | S4 | TFH | 5 | Tween 80 | 2:9 | No | No | 108.0 | 38 | +2.5 |
| 5 | S5 | TFH | 5 | Tween 80 | 3:8 | No | No | 98.0 | 43 | +10 |
| 6 | S6 | 2RPE | 5 | Tween 80 | 1:10 | No | No | 99.0 | 44 | -26 |
| 7 | S7 | RPE | 5 | Tween 80 | 2:9 | No | No | 73.5 | 42 | -25 |
| 8 | S8 | RPE | 5 | Tween 80 | 3:8 | No | No | 112 | 74 | -30 |
| 9 | D1 | TFH | 5 | Tween 80 | 61:9:1 | No | Yes | 134 | 24 | +15 |
| 10 | D2 | TFH | 5 | Tween 80 | 62:8:1 | No | Yes | 134 | 32 | +25 |
| 11 | D3 | TFH | 5 | Tween 80 | 63:7:1 | No | Yes | 136 | 38 | +17 |
| 12 | D4 | RPE | 5 | Tween 80 | 61:9:1 | No | Yes | 83 | 46 | +20 |
| 13 | D5 | RPE | 5 | Tween 80 | 62:8:1 | No | Yes | 95 | 79 | +20 |
| 14 | D6 | RPE | 5 | Tween 80 | 63:7:1 | No | Yes | 171 | 63 | +20 |
| 15 | C1 | RPE | 5 | Tween 80 | 71:9:1 | Yes | No | 128 | 47 | -25 |
| 16 | C2 | RPE | 5 | Tween 80 | 73:7:1 | Yes | No | 132 | 39 | -18 |
| 17 | C3 | RPE | 5 | Tween 80 | 75:5:1 | Yes | No | 132 | 37 | -19 |
| 18 | O1 | RPE | 5 | Tween 80 | 83:6:2:1 | Yes | Yes | 128 | 91 | -35 |
1
TFH: Thin Film Hydration
2
RPE: Reverse Phase Evaporation
3
LF: Lactoferrin
4
S: L ratio: surfactant: Lipid ratio
5
EE: Entrapment efficiency
6
Surfactant: DOTAP: SPC
7
Surfactant: SPC: cholesterol
8
Surfactant: SPC: DOTAP: cholesterol