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Study flow diagram for bacterial quantification. Flow diagram for the enrollment and progression of the patients in the present study. Time-points: T1, Prior to bonding; T2, 3 months after bonding; T3, 6 months after bonding; and T4, 18 months after bonding. T, total bacteria DNA; S, Streptococcus mutans DNA; L, Lactobacillus DNA; sIgA, secretory immunoglobulin A; MPO, myeloperoxidase; LDH, lactate dehydrogenase.
