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. 2019 Mar 18;17(5):4032–4038. doi: 10.3892/etm.2019.7408

Figure 6.

Figure 6.

miR-425-5p inhibitor upregulates pro-apoptotic gene expression in HeLa cells. (A) The protein expression levels of cytochrome c, caspase-3, caspase-9 and DRAM were determined by western blot analysis in HeLa cells following transfection with miR-425-5p inhibitor, inhibitor control, miR-425-5p inhibitor + AIFM1-siRNA or miR-425-5p inhibitor + control-siRNA. The mRNA expression levels of (B) cytochrome c, (C) caspase-3, (D) caspase-9 and (E) DRAM were determined by reverse transcription-quantitative polymerase chain reaction in HeLa cells following transfection with miR-425-5p inhibitor, inhibitor control, miR-425-5p inhibitor + AIFM1-siRNA or miR-425-5p inhibitor + control-siRNA. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01 vs. control group; #P<0.05 and ##P<0.01 vs. inhibitor group. DRAM, DNA damage regulated autophagy modulator 1; siRNA, small interfering RNA; control, untransfected HeLa cells; inhibitor control, HeLa cells transfected with inhibitor control; inhibitor, HeLa cells transfected with miR-425-5p inhibitor; inhibitor + control-siRNA, HeLa cells co-transfected with miR-425-5p inhibitor and control siRNA; inhibitor + siRNA, HeLa cells co-transfected with miR-425-5p inhibitor and AIFM1-siRNA.