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The expression of pCREB in ovarian tissue was examined by immunohistochemistry. (A) Strong expression was observed in the metformin + PDE4i group compared with (B) weak expression in the sham, PCOS control and metformin alone groups (pCREP; magnification, ×200). PCOS, polycystic ovary syndrome; PDE4i, phosphodiesterase 4 inhibitor; pCREP, phosphorylated cyclic AMP response element-binding protein.
