Figure 1.

Purification and identification of exosomes from the LNCaP cell line. (A) Transmission electron microscopy was performed to investigate exosome size and structure. Vesicles 30–150 nm in diameter were indicative of exosomes. (B) Particle size of exosomes ranged between 30 and 150 nm, with a peak of 72 nm, as assessed by NanoSight nanoparticle analysis. (C) Expression levels of CD9, Hsp70, Alix and GM130 were detected in exosomes by western blotting (lanes 1–2, exosomal pellets; lane 3, cells). (D) Expression levels of CD63 and CD81 were detected in exosomes by flow cytometry. Scale bar, 100 nm. CD, cluster of differentiation; GM130, Golgin subfamily A member 2; Hsp70, heat shock protein 70.