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. 2019 Mar 5;17(5):4463–4473. doi: 10.3892/ol.2019.10110

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effects of miR-375 mimics on osteoblast activity. (A) ALP staining. A large number of brown ALP particles was detected in the cytoplasm following transfection of osteoblasts with miR-375 mimics. Images were acquired under a microscope at ×400 magnification. (B) Alizarin red staining. Deposition of orange-red calcium nodules was observed post-transfection of osteoblasts with miR-375 mimics. Images were acquired under a microscope at ×400 magnification. (C) ALP activity and extracellular matrix mineralisation levels. (D) Cell proliferative activity. (E) Expression of osteoblast activity-associated marker genes. Scale bars, 25 µm. *P<0.05 and **P<0.01 vs. control. ALP, alkaline phosphatase; BSP, bone sialoproteinl; miR-375, microRNA-375; OB, osteoblast; OD, optical density; OPG, osteoprotegerin; OPN, osteopontin; RUNX2, runt-related transcription factor 2.