Restoration of ZEB1 expression reverses the suppressive effects of miR-508 on 786-O and A498 cell proliferation and invasion. (A) The relative protein expression level of ZEB1 was determined by western blot analysis in 786-O and A498 cells following co-transfection with miR-508 and pcDNA3.1 or pcDNA3.1-ZEB1 lacking the 3′-UTR of ZEB1. Cell (B) proliferation and (C) invasion were examined using CCK-8 and in vitro Transwell invasion assays, respectively, in 786-O and A498 cells following co-transfection with miR-508 and pcDNA3.1 or pcDNA3.1-ZEB1 lacking the 3′-UTR of ZEB1. *P<0.05 vs. miR-NC; #P<0.05 vs. miR-508 mimics + pcDNA3.1. ZEB1, zinc finger E-box binding homeobox 1; miR, microRNA; UTR, untranslated region; NC, negative control.