Figure 3.

Matrine promotes the nuclear Foxo3a localization and inhibits the levels of cytoplasmic Foxo3a. RC-4B/C cells were treated with increasing concentrations of matrine for 24 h. The nuclear proteins (A) and cytoplasmic proteins (B) were extracted as described in Materials and Methods. The levels of Foxo3 were determined by western blotting analysis. β-tubulin was used as cytoplasmic marker and lamin B1 was used as nuclear marker in RC-4B/C cells. The gray values of Foxo3a was shown in histogram in different concentrations of matrine-treated pituitary cancer cells. **P<0.01, compared with RC-4B/C cells without matrine.