Figure 1.

The expression of KLF2 is downregulated in liver cancer. A, Fluorescence quantitative polymerase chain reaction was used to detect the expression of Krüppel‐like factor 2 (KLF2) mRNA in liver tissues of 38 cases of liver cancer and in corresponding paracarcinomatous tissues; 18S rRNA served as the internal reference gene. B, Immunohistochemistry was used to detect the expression of KLF2 in liver cancer tissues and corresponding paracarcinomatous tissues of two random cases (scale bar: 50 μm, representative images). C‐D, Western blot was used to detect the protein level of KLF2 in hepatocellular carcinoma tissues and corresponding paracarcinomatous tissues of 14 cases. E, Western blot was used to detect the protein level of KLF2 in the mouse liver cancer model (Alb‐Cre; P53^fl/fl; Kras^G12D) and in control tissues, and the quantification was performed