Figure 2.

Krüppel‐like factor 2 (KLF2) inhibits liver cancer cell growth, migration and colony‐formation ability. A, MTT assay was used to study the effect of overexpressed KLF2 on liver cancer cell growth. B,C, Borden chamber experiment was used to study the effect of KLF2 overexpression on liver cancer cell migration (scale bar: 100 μm, representative images). D,E, Soft agar colony experiment was used to study the effect of KLF2 overexpression on the anchorage‐independent growth of cancer cells. F, MTT assay was used to study the effect of downregulating KLF2 on liver cancer cell growth. (G,H) Borden chamber experiment was used to study the effect of KLF2 downregulation on liver cancer cell migration (scale bar: 100 μm, representative images). I,J, Soft agar colony experiment was used to study the effect of KLF2 overexpression on the anchorage‐independent growth of cancer cells. *P < 0.05; **P < 0.01