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. 2019 Mar 4;110(4):1220–1231. doi: 10.1111/cas.13961

Figure 4.

Figure 4

Krüppel‐like factor 2 (KLF2) inhibits the Hedgehog/Gli1 signaling pathways in liver cancer cells. A, Luciferase reporter assay shows the effect of KLF2 expression on the Hedgehog/Gli1 signaling pathway. Cells were treated with Shh protein (100 ng/mL) for 8 h or co‐transfected (transient transfection, using Lipofectamine® 2000) with Gli1 expression vectors for 24 h before harvested. B, Western blot detected the effect of KLF2 expression on the Hedgehog/Gli1 signaling pathway and its downstream target genes. C,D, A soft agar colony‐formation experiment was used to detect the response of Gli1 expression to KLF2 biological function. E, Immunohistochemistry was performed to examine the expression of Gli, Patch and KLF2 in hepatocellular carcinoma tissues and non‐cancerous tissues (scale bar: 50 μm, representative images). *P < 0.05; **P < 0.01; ## P < 0.01. B‐D, Lentivirus was used to produce stable KLF2 and Gli1 transformants in 7404 and Huh7 cells