Table 3.

pre-miRNAs and mature miRNAs identified in A. angustifolia matching miRNA families in other plant species.

Loci		Pre-miRNA			miRNA 5P			miRNA 3P
miRNA family	miRNA locus	Length	Read counts	MFE	Sequence	Read count	Length	Sequence	Read count	Length
miR156	156	87	15,116	−48.5	TGACAGAAGAGAGTGAGCAC	13,367	20	GCTCACCATCTCTTTCTGTCAGC	1,583	23
miR159	159a	198	91,542	−71.8	CTTGGATTGAAGGGAGCTCC	77,010	20	AAGCTTCCTTCAGTCCAATCG	3	21
	159b	168	91,690	−91.2	AGCTCCCTTCGGTCCAATT	244	19	CTTGGATTGAAGGGAGCTCC	77,010	20
miR160	160	87	80	−47.1	TGCCTGGCTCCCTGTATGCCA	44	20	GTTGGCATAGAGGGAATCAAG	3	21
miR166	166a	71	1,008,365	−46.2	AAGGGGATTGCGGTCTGGCT	243	20	TCGGACCAGGCTTCATTCCCC	997,155	21
	166b	94	92,632	−42.6	GGACTGTTGTCTGGCTCGAAG	33	21	CCGGACCAGGCTTCATTCCCC	90,796	21
	166c	110	1,008,012	−54.5	GGAATGTTGTCTGGCTCGAGG	22	21	TCGGACCAGGCTTCATTCCCC	997,155	21
	166d	78	1,008,989	−47.7	GGAATGTTGTCTGGCTCGACT	780	21	TCGGACCAGGCTTCATTCCCC	997,155	21
	166e	96	1,063,754	−47.0	Non-detected	–	–	TCGGACCAGGCTTCATTCCCC	997,155	21
miR167	167a	120	13,848	−66.8	TGAAGCTGCCAGCATGATCTGA	11,024	22	AGATCATCTGGTAGCTTCAGC	580	21
	167b	117	4,785	−58.7	TGAAGCTGCCAGCATGATCTGG	2,611	22	TACCAGATCATGGTGGTGGCC	2	21
	167c*	91	4,788	−53.2	TGAAGCTGCCAGCATGATCTGG	2,611	22	AGGTCATCTGGCAGTTTCACC	6	21
	167d	109	4,783	−57.3	TGAAGCTGCCAGCATGATCTGG	2,611	22	TACCAGATCATGGTGGTGGCC	2	21
miR168	168	184	6,801	−74.0	TCGCTTGGTGCAGGTCGGGAA	4,436	21	CCCTGCTTGCATCAACTGAAT	332	21
miR169	169a	136	1,137	−99.7	AACAACTTGCCGGCTATTCTA	1	21	GGCAAGTTGTTCTCGGCTATG	918	21
	169b	131	195	−42.8	AAGCCAAGGATGAATTGCCGC	13	21	GGCAAGTTGTTCTTGGCTACG	72	21
	169c	155	1,802	−72.7	AAGCCAAGGATGATTTGCCGG	938	21	GGCAAGTTGTTCTTGGCTACG	72	21
	169d	158	2,886	−71.5	AAGCCAAGGATGATTTGCCGG	938	21	GGCAAGTTGTTCTCGGCTATG	918	21
miR171	171a	94	491	−50.4	GGATATTGGAGCGGTTCAACC	2	21	TTGAGCCGTGCCAATATCGCA	378	21
	171b	114	46	−63.1	GTGATGTTGGCTGGGCTCAAT	4	21	TGAGCCGTGCCAATATCACAA	16	21
	171c	84	36	−47.1	GTGATGTTGGCTGGGCTCAAT	4	21	TGAGCCGTGCCAATATCACAA	16	21
miR390	390a	83	1,998	−45.0	AAGCTCAGGAGGGATAGCGCC	1,952	21	CGCTATCTATCCTGAGCTTTT	13	23
miR394	394	80	611	−43.7	CTGGCATTCTGTCCACCTCC	312	21	AGGCGGACGGTATGCCAAGT	18	20
miR395	395a	112	2,712	−52.1	GTTCCCTCAACTACTTCAGAA	157	21	CTGAAGAGTTTGGGGGAACTC	2,157	21
	395b	95	2,514	−36.5	Non-detected	–	21	CTGAAGAGTTTGGGGGAACTC	2,157	21
miR396	396	120	5,246	−56.8	TTCCACAGCTTTCTTGAACTT	4,606	21	TTCAAGATTGCTGTGGGAAA	1	20
miR399	399a	114	136	−66.3	GGGGAGCTCTCCTTTGGCGGG	6	21	TGCCAAAGGAGAGTTGCCCTG	120	21
	399b	102	129	−64.0	GGGGGGCTCTCCTTTGGTGGG	2	21	TGCCAAAGGAGAGTTGCCCTG	120	21
	399c	111	134	−63.0	GGGGAGCTCTCCTTTGGCAGG	2	21	TGCCAAAGGAGAGTTGCCCTG	120	21
	399d	96	135	−53.2	GGGGAGCTCTCCTTTGGCGGG	6	21	TGCCAAAGGAGAGTTGCCCTG	120	21
	399e	112	134	−67.1	GGGGAGCTCTCCTTTGGCAGG	2	21	TGCCAAAGGAGAGTTGCCCTG	120	21
miR408	408	93	93	−55.6	GCCGGGAAGAGATAGCGCAT	1	20	TGCACTGCCTCTTCCCTGGCTG	79	22
miR529	529a	115	542	−51.9	AGAAGAGAGAGAGCACAGCCT	357	21	AGGCTGTGCTCTCTCTCTTC	1	21
	529b	89	544	−51.4	AGAAGAGAGAGAGCACAGCCT	357	21	GCTGTGCTCTCTCTCTTCTTC	2	21
	529c	119	540	−49.1	AGAAGAGAGAGAGCACAGCCT	357	21	Non-detected	–	–
	529d	104	540	−44.6	AGAAGAGAGAGAGCACAGCCT	357	21	Non-detected	–	–
	529e	103	463	−51.5	AGAAGAGAGAGAGTACAGCCC	35	21	GTTGTGCTCTCTCTCTTCTTC	383	21
miR1314	1314a*	71	6,878	−34.1	CTCCTACATTTAGGGTCGCCG	1,188	21	TCGGCCTTGAATGTTAGGAGAG	4,523	22
	1314b*	107	6,904	−53.5	CTCCTACATTTAGGGTCGCCG	1,188	21	TCGGCCTTGAATGTTAGGAGAG	4,523	22
	1314c*	135	5,853	−60.2	CTTCTAAATTTAAGGTCGCCG	560	21	TCGGCCTTGAATGTTAGGAGAG	4,523	22
	1314d*	124	5,921	−51.5	CTTCTAAATTTAAGGTCGCCG	560	21	TCGGCCTTGAATGTTAGGAGAG	4,523	22

^*Precursor miRNAs also identified in Araucaria cunninghamii.