Table 3.
Bacterial strains and plasmids.
| Strain or plasmid | Genotype | Resource |
|---|---|---|
| Escherichia coli | ||
| DH5α | F– φ80ΔlacZM15 Δ(lacZYA-argF)U169 deoP recA1 endA1 hsdR17 (rK-mK-) | Sambrook and Russell, 2001 |
| BL21(DE3)pLysS | F′- ompT gal[dcm][lon] hsdsB (rB- mB-; an E. coli B strain) with DE3 and pLysS | Studier and Moffatt, 1986 |
| Azotobacter vinelandii | ||
| DJ | Highly transformable variant of OP | Setubal et al., 2009 |
| UW394 | ΔmosBA::spc | This study |
| UW466 | ΔvnfDGK::tet→ ; ΔanfDGK::kan→ | This study |
| UW463 | ΔmosBA::spc; ΔvnfDGK::tet→ ; ΔanfDGK::kan→ | This study |
| UW356 | ΔnifA::spc | Poza-Carrion et al., 2014 |
| DJ33 | ΔnifDK | Robinson et al., 1986 |
| Plasmids | ||
| pBluescript KS (+) | Cloning vector | Agilent |
| pGEMT-vector | Cloning vector | Promega |
| pET28a (+) | Expression vector | Novagen |
| pUC4K | Vector containing Kan resistance cassette | Pharmacia |
| pBBR1-MCS3 | Vector containing Tet resistance cassette | Obranic et al., 2013 |
| pHP45Ω | Vector containing Spc/Sm resistance cassette | Prentki and Krisch, 1984 |
| pRHB268 | pGEMT carrying mosBA flanking regions and spc cassette | This study |
| pN2MN14 | pBSKS(+) carrying anfDGK flanking regions and kan cassette | This study |
| pN2MN18 | pBSKS(+) carrying vnfDGK flanking regions and tet cassette | This study |
| pN2MN72 | mosBA genes cloned into pET28a (+) | This study |