. 2019 Mar 26;7:54. doi: 10.3389/fbioe.2019.00054

Table 2.

Box-Behnken Design of RSM for optimization of carbon and nitrogen concentration in cultivation media of C. oleaginosus.

Run	C:N	X1	X2	Biomass	Lipid weight	Lipid content	Lipid yield
		(C (g. L⁻¹))	(N (g. L⁻¹))	(g. L⁻¹)	(g. L⁻¹)	(% g. g⁻¹)	(g lipid. g⁻¹ C)
1	60	8	0.13	9.75 ± 1.09	3.25 ± 0.09	33.78 ± 4.68	0.04 ± 0.01
2	30	8	0.26	9.5 ± 0.11	1.82 ± 0.14	19.15 ± 1.53	0.23 ± 0.02
3	12	8	0.67	10.15 ± 0.13	1.39 ± 0.10	13.71 ± 1.10	0.17 ± 0.01
4	120	16	0.13	15.1 ± 0.60	6.69 ± 0.22	44.36 ± 2.56	0.42 ± 0.01
5	60	16	0.26	15.1 ± 0.41	3.18 ± 0.07	21.06 ± 0.48	0.20 ± 0.00
6	24	16	0.67	17.35 ± 1.10	2.33 ±0.29	13.48 ± 1.90	0.15 ± 0.02
7	180	24	0.13	16.85 ± 1.33	6.97 ± 0.76	41.66 ± 5.74	0.29 ± 0.03
8	90	24	0.26	19.7 ± 2.24	7.38 ± 1.01	38.09 ± 8.28	0.31 ± 0.04
9	36	24	0.67	23.95 ± 2.36	3.78 ± 0.61	15.98 ± 3.40	0.16 ± 0.02
10	240	32	0.13	15.4 ± 0.14	4.1 ± 0.47	26.64 ± 3.21	0.13 ± 0.01
11	120	32	0.26	22.9 ± 0.10	6.29 ± 0.73	27.48 ± 3.23	0.20 ± 0.02
12	48	32	0.67	24.3 ± 0.55	5.85 ± 0.14	24.09 ± 0.80	0.18 ± 0.00

Elemental weight ratio of carbon to nitrogen were delivered by glucose and yeast extract, respectively.