Figure 4.

Psoriatic fibroblasts support pro-inflammatory activation of macrophages. (A) TNF-α and IL-10 levels in THP-1 derived macrophages incubated during 24 h with conditioned media from IL-1β-stimulated psoriatic fibroblast (PFCM) (n = 4 biopsies) or IL-1β-stimulated healthy fibroblasts (HFCM) (n = 4 biopsies). When indicated, healthy fibroblasts where pretreated with 10 μM indomethacin (Indo) before stimulation. Data represent mean ± SD. **p < 0.01, ****p < 0.0001 respect untreated macrophages (B); +++p < 0.001 ++++p < 0.0001 vs. macrophages treated with HFCM using Tukey's multiple comparison test. (B) TNF-α and IL-10 levels in THP-1 derived macrophages treated during 24 h with PGE₂ (30 ng/ml), indomethacin (10 μM) or their combination in the presence or the absence of IL-1β (2.5 ng/ml). Data represent mean ± SD (n = 6). ***p < 0.001, ****p < 0.0001 vs. untreated cells (B). +++p < 0.001 respect IL-1β-treated cells using Tukey's multiple comparison test. All conditions were assayed in duplicate.