Figure 2.

Representative pillar deflections and corresponding TRPV4-mediated currents. (A) Bright-field image of a single HEK-293T cell expressing TRPV4 cultured on the pillar array, scale bar 10 μm. (B) Series of mechanical stimuli are applied directly at cell-substrate interface by deflecting the pilus subjacent to the cell (orange box). Middle panel shows the movement of indicated pilus in response to the stimuli of increasing magnitude from 296 to 667 nm. (C) The center point of the pilus is determined from a 2D Gaussian fit of intensity values in the images of before and during deflection. (D) Representative traces of TRPV4-mediated currents corresponding to the stimuli presented in (B).