Figure 1.

Flu inhibited viabilities of breast cancer cell lines. A. Chemical structure of Flu. B. Inhibitory effects of Flu on the viabilities of several breast cancer cell lines after 72 hours of treatment (IC₅₀ values, μM). C. Dose- and concentration-dependent inhibitory effects of Flu on cell viabilities in two TNBC cell lines (4T1 and MDA-MB-231). MTT assays were used to measure cell viabilities after Flu treatment for 48 and 72 hours, respectively. Viabilities of vehicle treated cells at each time point were counted as 100%. D and E. Inhibitory effects of Flu on 4T1, 4T1-Fu5, MDA-MB-231 and MDA-MB-231-Fu10 cell colony formation. Quantifications are shown in the right for each cell line. Images shown are representatives from three independent experiments. F. Flu treatment decreased phosphorylation of ERK1/2 and AKT in 4T1 and MDA-MB-231 cell lines. Molecular weight of bands on Markers are shown on the right of each image (kilo Dalton, kD). β-actin served as the internal control. Images shown are representatives from three independent experiments. G. Relative expression of phosphorylation of ERK1/2 and AKT were quantified using Image J software and normalized to β-actin expression. *P<0.05; **P<0.01; ***P<0.001.