Figure 7.

Effects of Flu on lung metastasis, liver metastasis and bone metastasis in mouse models. A and B. Flu strongly inhibited spontaneous lung metastasis. Lungs were harvested in the subcutaneous xenograft model. Then the total numbers of lung metastases were counted with eyes. A. Blue arrows indicate the metastasis on the surface of the lung. B. Quantified values were shown in the right. ***P<0.001. C and D. Flu didn’t inhibit the growth of bone metastasis. 2.5 × 10⁴ luciferase-expressing 4T1 cells in 10 μL sterile HBSS were injected into the tibial growth plate on the back left leg to establish experimental liver metastasis model. The growth of bone metastasis was measured via IVIS every week. E and F. Flu didn’t show inhibition on liver metastasis. 25 × 10⁴ luciferase-expressing 4T1 cells in 25 μL sterile HBSS were injected into the tip of the exposed spleen to establish experimental liver metastasis model. Then the growth of liver metastasis was measured via IVIS every week.