Figure 2.

SDHA was sensitive to chidamide and its ectopic expression was related to disease development in MM. A. RNA was isolated from normal volunteers and MM patients. cDNA used in Realtime RT PCR was reverse transcribed from the RNA above. The six most differentially expressed genes were validated by Real-time RT PCR. After adding 6 μM chidamide, the expression of SDHA and FCER2 was increased, and MRPL30 decreased in patients’ BMMCs (n = 3) compared with cells adding DMSO, (C stood for cells with chidamide treatment). B. mRNA levels of the six most differentially expressed genes between normal volunteers (n = 3) and MM patients (n = 3) was detected by Realtime RT PCR. The expression of SDHA was upregulated and ITGA7 was downregulated in MM patients (N stood for normal volunteers; M stood for MM patients). C. RNA was isolated from normal volunteers (n = 9), MGUS (n = 13) and MM patients (n = 23). By Realtime RT PCR, SDHA expression in normal volunteers was the highest and followed by patients with MGUS and initial MM. Patients with relapse MM (n = 10) had the lowest SDHA expression.