Figure 5.

Bortezomib and lenalidomide had synergistic effect with chidamide via SDHA. CCK8 assay was used to detect the synergistic effect between agents. Different concentrations of agents were added to wells respectively for 24 h. CCK8 was used as described in Materials and Methods. A. Left panel showed 0.025 g/L bortezomib and 0.6 μM chidamide have no synergistic effect and not regulated by SDHA siRNA. Right panel showed 0.0025 g/L bortezomib and 0.6 μM chidamide have synergistic effect (calculated as described in materials and methods). But the regulation effect of SDHA siRNA was not significant. B. 0.125 g/L and 0.0125 g/L lenalidomide both have synergistic effect with chidamide. And this synergistic effect was reversed by SDHA siRNA. C. CI distribution between bortezomib and chidamide in different inhibition rate (FA). D. CI distribution between lenalidomide and chidamide in different inhibition rate (FA).