Figure 6.

Chidamide decreased ROS production through increasing the expression of SDHA. A. Chidamide could increase the level of SDH in H929 cells showed by realtime RT PCR. B. Western blot showed that after knocking down SDHA by siRNA in H929 cells, the level of HIFα protein increased. Chidamide could increase the expression of SDHA and decrease the level of HIFα. But when SDHA knocked down, chidamide had little effect on HIFα expression. The quantifications of HIFα expression were shown in the right panel. C. Mitochondrial ROS production was determined by CM-H2DCFDA staining and flow cytometry. The production of ROS was decreased after knocking down SDHA in H929 cells. Chidamide decreased the production of ROS. But when SDHA knocked down, chidamide had little effect on ROS production. D. BMMCs of patients with MM produced more ROS than normal volunteers.