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. 2019 Mar 1;9(3):496–510.

Figure 7.

Figure 7

UCA1 acts as a miR-590-3p sponge. A, B. Overexpression of UCA1 in KRAS-independent PDAC cell lines (PaTu8988 and PANC-1 cells) promotes the expression of KRAS protein and mRNA. C. qRT-PCR analysis of KRAS mRNA levels in Mpanc96 and HPAF-II cells with UCA1 downregulation. D. Prediction of potential miRNAs targeting UCA1 and binding to the KRAS 3’UTR by bioinformatics analysis (miRanda; http://www.micro-RNA.org/). E. The bioinformatics prediction of the miR-590-3p binding sequence in UCA1 is shown. F, G. qRT-PCR analysis of miR-590-3p levels in PDAC cell lines with UCA1 silencing or overexpression. H. Relative expression of UCA1 after miR-590-3p upregulation in Mpanc96 and HPAF-II cells. I. The relative luciferase activity was measured by a dual luciferase reporter assay after the cotransfection of wild-type or mutant UCA1 and miR-590-3p.