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. 2019 Mar 1;9(3):585–596.

Figure 1.

Figure 1

MiR-124 downregulation is inversely associated with prognosis and MGAT5 expression in BC. A: qRT-PCR analyses of MGAT5 mRNA levels in BC and normal tissues (n=20). B: Western blot analyses of MGAT5 protein expression in T and N tissues. β-actin was used as the internal control. C: miR-124, miR-101-3p and their deductive domains in the 3’-UTR of MGAT5. D: Luciferase reporter assay of the connection between the 3’UTR of MGAT5 and miR-124 in 293T cells; E: qRT-PCR analysis of MGAT5 mRNA expression level after infected miR-124 mimics or inhibitor in MDA-MB-231 and MCF-7 cells. F: Western blot analyses of MGAT5 protein expression after infected miR-124 mimics or inhibitor in MDA-MB-231 and MCF-7 cells. β-actin was used as the internal control. G: qRT-PCR analyses of miR-124 levels in BC and normal tissues (n=20). H: Kaplan-Meier survival curves of OS based on miR-124 expression in 1262 BC patients using the online bioinformatics tool Kaplan-Meier plotter (METABRIC); I: Spearman’s correlation assay showed that miR-124 was negatively correlated with MGAT5 mRNA expression in BC specimens. *P<0.05, **P<0.01.