Table 2.
Sequence of the primers in this study
| Pimer | Sequence |
|---|---|
| MGAT5 | Forward Primer AGGGCCATCCTGGGTTCATTA |
| Reverse Primer AAACTGCTGCGGGTTCAGATTC | |
| β-actin | Forward Primer CTGGTGCCTGGGGCG |
| Reverse Primer AGCCTCGCCTTTGCCGA | |
| miR-124 | Forward Primer CGACGTAAGGCACGCG |
| Reverse Primer CAGTGCAGGGTCCGAGGTAT | |
| RT-Primer GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGCATTCAC | |
| miR-101 | Forward Primer CGACGTACAGTACTGTG |
| Reverse Primer CAGTGCAGGGTCCGAGGTAT | |
| RT-Primer GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTTCAGTTAT | |
| U6 | Forward Primer CGAGCACAGAATCGCTTCA |
| Reverse Primer CTCGCTTCGGCAGCACATAT | |
| RT-Primer CGAGCACAGAATCGCTTCACGAATTTGCGTGTCAT | |
| miR-124 mimic | UAAGGCACGCGGUGAAUGCC |
| miR-124 inhibitor | GGCAUUCACCGCGUGCCUUA |
| miR-101 mimic | UACAGUACUGUGAUAACUGAA |
| miR-101 inhibitor | UUCAGUUAUCACAGUACUGUA |
| Inhibitor NC | CAGUACUUUUGUGUAGUACAA |
| Mimic NC | UUGUACUACACAAAAGUACUG |
| Dual luciferase vector plasmid | GP-miRGLO |
| MGAT5 3’UTR | WT AGTAATGCAG GATTCTGCAA ACAAGGTGTC GCCGTCCAAA TGTACTGTCC TGGCATAGAG |
| miR-101 | MUT AGTAATGCAG GATTCTGCAA ACAAGGTGTC GCCGTCCAAA TCATGACACC TGGCATAGAG |
| MGAT5 3’UTR | WT GGAGATATTA GTCAGTTTCT TTAGTGATAT TTGTTTCCTT GATGTGCCTT TTCGTTTTTC |
| miR-124 | MUT GGAGATATTA GTCAGTTTCT TTAGTGATAT TTGTTTCCTT GATCACGGAA TTCGTTTTTC |
| RNAi Lentiviral Vector | pGMLV-SC5 |
| Sh-NC | Primer-T gatcTGTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTTTc |
| Sh-NC | Primer-B aattgAAAAAATTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACGTTCGGAGAACa |
| sh-MGAT5 | Primer-T GATCCGGCGGAAATTCGTACAGATTTCAAGAGAATCTGTACGAATTTCCGCCTTTTTTG |
| sh-MGAT5 | Primer-B AATTCAAAAAAGGCGGAAATTCGTACAGATTCTCTTGAAATCTGTACGAATTTCCGCCG |
| Lentiviral Vector | pGMLV-MI7 |
| miR-124 inhibitor | Primer-F GATCCGACGGCGCTAGGATCATCAACGGCATTCACCATCTGCGTGCCTTACAAGTATTCTGGTCACAGAATACAACGGCATTCACCATCTGCGTGCCTTACAAGATGATCCTAGCGCCGTCTTTTTTG |
| miR-124 inhibitor | Primer-R AATTCAAAAAAGACGGCGCTAGGATCATCTTGTAAGGCACGCAGATGGTGAATGCCGTTGTATTCTGTGACCAGAATACTTGTAAGGCACGCAGATGGTGAATGCCGTTGATGATCCTAGCGCCGTCG |
| Lentiviral Vector | PGMLV-6395 |
| MGAT5 | Primer-F CCGCTCGAGGCCACCATGGCTCTCTTCA |
| MGAT5 | Primer-R CCGGACGCGTCTATAGGCAGTCTTTGCAGAGAGC |
| miR-124 | Primer-F CCGGAATTCGGATTCAGGGCGAACCAACTG |
| miR-124 | Primer-R CCGCTCGAGGCACAGGCGGGAACTACTGC |