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. 2019 Mar 7;41(5):2833–2843. doi: 10.3892/or.2019.7053

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Copyright: © Zheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — PD-L1 overexpression promotes the proliferation of HNSCC cell lines. (A) HNSCC cell proliferation was detected with Cell Counting Kit-8 assays, **P<0.01 vs. PD-L1^over ^NC, ^##P<0.01 vs. PD-L1^{RNAi NC}. (B) HNSCC cells were labeled with Cell-Light™ EdU (red), and nuclei were stained by Hoechst 33342 (blue). Histograms represent the positive rate of EdU staining. PD-L1 overexpression promotes the proliferation of HNSCC cell lines. (C) Colony formation assay. Cell colony numbers (>50 cells/unit) were counted. Data were expressed as the mean ± standard deviation. **P<0.01. PD-L1, programmed death-ligand 1; HNSCC, head and neck squamous cell carcinoma; PD-L1^over, PD-L1-overexpressing; PD-L1^RNAi, PD-L1 knockdown; NC, negative control.

Figure 3. — PD-L1 overexpression promotes the proliferation of HNSCC cell lines. (A) HNSCC cell proliferation was detected with Cell Counting Kit-8 assays, **P<0.01 vs. PD-L1^over ^NC, ^##P<0.01 vs. PD-L1^{RNAi NC}. (B) HNSCC cells were labeled with Cell-Light™ EdU (red), and nuclei were stained by Hoechst 33342 (blue). Histograms represent the positive rate of EdU staining. PD-L1 overexpression promotes the proliferation of HNSCC cell lines. (C) Colony formation assay. Cell colony numbers (>50 cells/unit) were counted. Data were expressed as the mean ± standard deviation. **P<0.01. PD-L1, programmed death-ligand 1; HNSCC, head and neck squamous cell carcinoma; PD-L1^over, PD-L1-overexpressing; PD-L1^RNAi, PD-L1 knockdown; NC, negative control.