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. 2019 Mar 7;41(5):2833–2843. doi: 10.3892/or.2019.7053

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Copyright: © Zheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — PD-L1 enhances the sensitivity of HNSCC cells to mTOR inhibitor in vitro. (A) HNSCC cells were cultured with rapamycin and cell proliferation was detected by Cell Counting Kit-8 assay (B) and the proliferation inhibition rate was calculated. (C) Colony formation assay results. Cell colony numbers (>50 cells/unit) were counted *P<0.05 and **P<0.01. PD-L1, programmed death-ligand 1; HNSCC, head and neck squamous cell carcinoma; mTOR, mammalian target of rapamycin; RP, rapamycin; PD-L1^over, PD-L1-overexpressing; PD-L1^RNAi, PD-L1 knockdown; NC, negative control.

Figure 6. — PD-L1 enhances the sensitivity of HNSCC cells to mTOR inhibitor in vitro. (A) HNSCC cells were cultured with rapamycin and cell proliferation was detected by Cell Counting Kit-8 assay (B) and the proliferation inhibition rate was calculated. (C) Colony formation assay results. Cell colony numbers (>50 cells/unit) were counted *P<0.05 and **P<0.01. PD-L1, programmed death-ligand 1; HNSCC, head and neck squamous cell carcinoma; mTOR, mammalian target of rapamycin; RP, rapamycin; PD-L1^over, PD-L1-overexpressing; PD-L1^RNAi, PD-L1 knockdown; NC, negative control.