Figure 5.

PLCε regulates LDHA through the STAT3 pathway. (A) PLCε knockdown downregulated phosphorylation of STAT3 in T24 cells. (B) After 24 h post treatment of STAT3 inhibitor stattic (5 and 10 µM dissolved in DMSO), LDHA expression was downregulated in T24 cells with STAT3 phosphorylation inhibition. (C) IL-6 (20 ng/ml for 24 h) rescued PLCε knockdown mediated STAT3 phosphorylation and LDHA downregulation in T24 cells. Values were presented as means ± standard deviations of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001, compared with the (A) sh-NC group, (B) DMSO group or (C) sh-PLCε group. PLCε, phosphatidylinositol-specific phospholipase Cε; LDHA, lactate dehydrogenase; STAT3, signal transducer and activator of transcription 3; sh, short hairpin; NC, negative control; DMSO, dimethyl sulfoxide; p-, phospho-; IL, interleukin.