Figure 6.

STAT3 regulates LDHA expression through binding to its promoter. (A) ChIP results using 3 pairs of primers (ChIP 1, 2 and 3) targeting 3 binding sites predicted by JASPAR. (B) Model of the LDHA promoter luciferase reporter plasmid and its mutants. (C) Dual luciferase reporter assay using STAT3 overexpression plasmid and different LDHA promoter luciferase reporter mutant plasmids in HeLa cells. Values were presented as means ± standard deviations of three independent experiments. ***P<0.001, compared with the ChIP 1 and pGL4-pLDHA+pcDNA3.1 group. LDHA, lactate dehydrogenase; STAT3, signal transducer and activator of transcription 3; ChIP, chromatin immunoprecipitation.