Skip to main content
. 2019 Mar 7;41(5):2689–2702. doi: 10.3892/or.2019.7054

Figure 1.

Figure 1.

Lentivirus-shPLCε and GANT61 inhibit the protein and mRNA expression level of PLCε and Gli-1/Gli-2. (A) PLCε was highly expressed in the PCa (LNCaP) and CRPC (22RV1 and EN-R) cell lines. Gli-1/Gli-2 and AR expression levels were higher in CRPC (22RV1 and EN-R) cells compared to PCa (LNCaP) cells as shown by western blotting. (B) The mRNA expression levels of PLCε, Gli-1/Gli-2 and AR in LNCap, 22RV1 and EN-R cells were detected by RT-qPCR (*P<0.05, **P<0.01 and ***P<0.001). (C) PLCε knockdown was induced by transfecting lentivirus (LV)-shPLCε into CRPC cell line. Total cellular proteins were detected by western blotting. (D) Relative PLCε mRNA expression level was determined by RT-qPCR. β-actin was used as an internal control (**P<0.01 and ***P<0.001 compared with the blank control). (E-G) GANT61 inhibited the protein and mRNA expression level of Gli-1/Gli-2. β-actin was used as an internal control (**P<0.01 compared with the blank control). PLCε, phospholipase Cε; Gli, glioma-associated homolog; PCa, prostate cancer; CRPC, castration-resistant PCa; EN-R, enzalutamide-resistant cell line; AR, androgen receptor; RT-qPCR, reverse transcription quantitative polymerase chain reaction.