Figure 4.

CD1d⁺DCs highly express MHCs and costimulatory molecules, revealing a stronger ability to present antigens to T cells. (A) The gating strategies of flow cytometric analysis and flow cytometric analysis of CD40, CD80, CD86, MHC-I/II expression on splenic CD1d⁺DCs and CD1d⁻DCs. The splenic cells were isolated from the tumor-bearing model (day 28). (B) Flow cytometric analysis of CD40, CD80, CD86 and MHC-I/II expression levels in human lung cancer tissues from lung cancer patients, n=4. Data presented in A and B were pooled from three independent experiments each with 3–4 mice and expressed as the mean ± SEM. **P<0.01, ***P<0.001. DCs, dendritic cells; MFI, median fluorescence intensity; SEM, standard error of the mean; FSA, FSC-A (area of FSC); SSC, SSC-H (height of SSC).