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. 2019 Mar 18;41(5):2818–2832. doi: 10.3892/or.2019.7077

Figure 5.

Figure 5.

RT-qPCR and western blotting of MCF-7 cell extracts. GAPDH was used as a loading control. (A) Ad-VT and Ad-VP3 inhibited autophagy-mediated gene expression. The mRNA expression level of the genes encoding Beclin-1, LC3 and P62 were detected in MCF-7 cells using RT-qPCR. (B) Western blot analysis of LC3 expression in MCF-7 cells following infection with adenoviruses at various durations (6, 12, 24 and 48 h), or with different concentrations of adenoviruses (1, 10, and 100 MOI) for 6 h. (C) Western blot analysis of Beclin-1 expression in MCF-7 cells. (D) Western blot analysis of P62 expression in MCF-7 cells. Data are presented as the means ± standard deviation, n=3. *P<0.05, **P<0.01, ***P<0.001 vs. the control group. Ad-VP3, Ad-Apoptin; Ad-VT, Ad-Apoptin-hTERTp-E1a; LC3, microtubule-associated protein 1A/1B-light chain 3; MOI, multiplicity of infection; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.