Table 5.

Effects of Mn preconditions diet 96 h post-hatch and replacing of manganese sulfate with Bioplex Mn on plasma and liver enzyme activity and expression of jejunum divalent metal transfer 1 mRNA expression at 21 D.

Item	Plasma T-SOD (U/ml)	Liver ALT (U/L)4	DMT1
Mn preconditioning period (MnPP)
MnPD1 (Mn preconditions diet)	52.86	898	1.03
NPCD (non-preconditioning diet)	54.26	932	0.98
Mn source
Basal diet3	52.74	847	1.02
Manganese sulfate2	53.70	963	0.99
Bioplex Mn	53.80	905	1.02
Mn level (mg/kg)2
12	54.99	918	1.01
60	52.45	950	0.99
Pooled SE
	P-values
MnPP	0.570	0.699	0.289
Mn source	0.680	0.590	0.778
Mn level	0.454	0.723	0.706
MnPP × source	0.666	0.167	0.370
MnPP × level	0.060	0.769	0.895
Mn Source × level	0.698	0.276	0.226
MnPP x source × level	0.3093	0.676	0.316
SEM	0.990	40.707	0.170

T-SOD – Total superoxide dismutase, ALT – alanine transaminase.

DMT1 – divalent metal transfer 1.

¹No supplemental Mn in the basal diet fed to broiler chicks for 96 h.

²Data represent the means of 24 replicates cages.

³Data represent the means of 12 replicates cages.

⁴U/L: one unit catalyzes the formation of 1 μmole per liter of NAD per minute at 25°C.