Table 1.

Ingredient and nutrient compositions of the experimental diets.

Diet
	1	2	3	4	5	6	7
Ingredients				%
Yellow corn1	66.43	66.43	66.43	66.43	66.43	66.43	66.43
SBM1	20.30	20.30	20.30	20.30	20.30	20.30	20.30
CaCO32	10.60	10.39	10.20	10.60	10.60	10.60	10.60
Soy oil	1.00	1.00	1.00	1.00	1.00	1.00	1.00
NaCl	0.28	0.28	0.28	0.28	0.28	0.28	0.28
Vit-Min3	0.30	0.30	0.30	0.30	0.30	0.30	0.30
NaHCO3	0.15	0.15	0.15	0.15	0.15	0.15	0.15
DL Met	0.14	0.14	0.14	0.14	0.14	0.14	0.14
BioLys 65%	0.06	0.06	0.06	0.06	0.06	0.06	0.06
Silica4	0.65	0.33	–	0.65	0.64	0.64	0.63
Monodicalcium phosphate (MDCP)5	0.10	0.63	1.15	0.10	0.10	0.10	0.10
Phytase6	–	–	–	0.00	0.01	0.01	0.02
Total	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Nutrients7
ME, kcal/kg	2800.00	2800.00	2800.00	2800.00	2800.00	2800.00	2800.00
CP, %	15.00	15.00	15.00	15.00	15.00	15.00	15.00
Dig Lys, %	0.68	0.68	0.68	0.68	0.68	0.68	0.68
Dig Met, %	0.35	0.35	0.35	0.35	0.35	0.35	0.35
Dig SAA, %	0.55	0.55	0.55	0.55	0.55	0.55	0.55
Dig Thr, %	0.48	0.48	0.48	0.48	0.48	0.48	0.48
Ca, %	4.20	4.20	4.20	4.20	4.20	4.20	4.20
P, %	0.35	0.46	0.57	0.35	0.35	0.35	0.35
NPP, %	0.120	0.230	0.340	0.120	0.120	0.120	0.120
Phytase, FYT/kg	–	–	–	300.00	600.00	1200.00	1800.00
Phytase found, FYT/kg	BDL	BDL	BDL	345.30	580.60	1350.00	1900.40

¹Total P analyzed with the official AOAC (2007) methodology.

²50% particle size between 3 to 5 mm (particulate) and 50% particle size < 3 mm (ground).

³Vitamin-mineral premix contained the following by kg of feed: vitamin A (12,000 IU), vitamin D₃ (3,500 UI), vitamin E (30 mg), vitamin K₃ (3.5 mg), thiamine (2.5 mg), riboflavin (7.0 mg), pyridoxine (3.5 mg), vitamin B₁₂ (0.02 mg), niacin (30 mg), pantothenate (10 mg), folate (1.0 mg), biotin (0.1 mg), choline (300 mg), copper (15 mg), iron (60 mg), manganese (110 mg), iodine (1.0 mg), zinc (80 mg), and selenium (0.3 mg).

⁴Inert filler; SiO_2.

⁵Total Ca and P analyzed with the official AOAC (2007) methodology; 21% P, 17% Ca.

⁶ Citrobacter Braakii phytase; Ronozyme® HiPhos 10,000 FYT/g. DSM Nutritional Products.

⁷Formulated from analyzed corn and SBM values for AA, protein, and phytic P with NIR technology (AminoDAT Platimun Versión 5.0, Evonik industries).