Altered RNAP III activity affects carbon flux. Low activity of RNAP III in rpc128-1007 strain is correlated with decreased carbon flow through glycolysis in comparison with reference strain. In contrast, maf1Δ cells demonstrate increased carbon flow through hexokinase step and lower glycolysis compared with the control strain. In maf1Δ, excess glucose-6-P (G6P) is redirected into PPP and trehalose and glycogen biosynthesis. As a result, fructose 1,6-bisphosphate (F16BP) concentration decreases in maf1Δ. From the increased glycerol concentration, carbon flux is partially redirected towards upper glycolysis at PEP. Green: decrease in carbon flux; Red: increase in carbon flux. Legend: glucose-6-phosphate (G6P); fructose-6-phosphate (F6P); fructose 1,6-bisphosphate (F16BP); dihydroxyacetone phosphate (DHAP); glyceraldehyde-3-phosphate (G3P); 6-phosphogluconate (6PG); ribose-5-phosphate (R5P); 3-phosphoglyceric acid (3PG); phosphoenolpyruvate (PEP).