Table 1.
Materials and technological parameters of Various formulation
| sample | Lipid (mg) | Lipid surfactant (ML, Mg) | Water surfactant (ML, Mg) | Water added (ML) | PS (nm) | PDI | Zeta potential (mV) | EE (%) | DL (%) | |
|---|---|---|---|---|---|---|---|---|---|---|
| Before lyophilization | SLN1 without doxycycline | Palm oil (600) | Lecithin (60) | Tween 80 (6) | 50 | 251 ± 15 | 0.277 ± 0.021 | −31.6 ± 1.4 | 95.4 ± 3.5 | 11.3 ± 1.1 |
| SLN 1 with doxycycline | Palm oil (600) | Lecithin (60) | Tween 80 (6) | 50 | 265 ± 23 | 0.284 ± 0.012 | −29.3 ± 2.1 | |||
| SLN 2 without doxycycline | Stearic acid (600) | Span 80 (6) | Poloxamer407 (60) | 100 | 456 ± 21 | 0.429 ± 0.032 | −11.6 ± 4.4 | 93.7 ± 5.3 | 8.9 ± 2.3 | |
| SLN 2 with doxycycline | Stearic acid (600) | Span 80 (6) | Poloxamer407 (60) | 100 | 465 ± 23 | 0.344 ± 0.012 | −14.3 ± 3.5 | |||
| SLN 3 without doxycycline | Palm oil (1200) | Span 80 (6) | Tween 80 (3) | 100 | 358 ± 39 | 0.536 ± 0.019 | −9.6 ± 1.7 | 94.1 ± 7.1 | 9.2 ± 3.8 | |
| SLN 3 with doxycycline | Palm oil (1200) | Span 80 (6) | Tween 80 (3) | 100 | 375 ± 42 | 0.591 ± 0.010 | −11.3 ± 2.6 | |||
| SLN 4 without doxycycline | Stearic acid (1200) | Lecithin (60) | Tween 80 (3) | 50 | 491 ± 25 | 0.447 ± 0.033 | −18.6 ± 1.4 | 97.4 ± 4.5 | 10.7 ± 1.4 | |
| SLN 4 with doxycycline | Stearic acid (1200) | Lecithin (60) | Tween 80 (3) | 50 | 415 ± 59 | 0.494 ± 0.093 | −19.3 ± 1.2 | |||
| After lyophilization | SLN 1 without doxycycline | Palm oil (600) | Lecithin (60) | Tween 80 (6) | 50 | 294 ± 27 | 0.281 ± 0.044 | −30.7 ± 2.04 | 94.9 ± 3.2 | 11.2 ± 1.3 |
| SLN 1 with doxycycline | Palm oil (600) | Lecithin (60) | Tween 80 (6) | 50 | 299 ± 34 | 0.290 ± 0.027 | −28.7 ± 3.2 | |||
| SLN 2 without doxycycline | Stearic acid (600) | Span 80 (6) | Poloxamer407 (60) | 100 | 491 ± 33 | 0.499 ± 0.042 | −12.6 ± 3.5 | 93.9 ± 4.7 | 8.2 ± 1.9 | |
| SLN 2 with doxycycline | Stearic acid (600) | Span 80 (6) | Poloxamer407 (60) | 100 | 471 ± 34 | 0.412 ± 0.012 | −14.3 ± 3.5 | |||
| SLN 3 without doxycycline | Palm oil (1200) | Span 80 (6) | Tween 80 (3) | 100 | 378 ± 28 | 0.591 ± 0.053 | −17.6 ± 2.1 | 91.3 ± 2.7 | 8.8 ± 3.1 | |
| SLN 3 with doxycycline | Palm oil (1200) | Span 80 (6) | Tween 80 (3) | 100 | 399 ± 39 | 0.612 ± 0.125 | −15.3 ± 1.7 | |||
| SLN 4 without doxycycline | stearic acid (1200) | Lecithin (60) | Tween 80 (3) | 50 | 511 ± 45 | 0.493 ± 0.325 | −17.8 ± 1.7 | 96.1 ± 3.9 | 9.9 ± 1.7 | |
| SLN 4 with doxycycline | stearic acid (1200) | Lecithin (60) | Tween 80 (3) | 50 | 575 ± 39 | 0.524 ± 0.987 | −16.3 ± 2.2 |
PS particle size, PDI polydispersity index, EE entrapment efficiency, DL drug loading