The data were expressed as fold changes of relative values of mRNA levels in each of SCI groups vs. the SI group whereas the statistical analysis was performed using the mRNA expression ratio of TRPV1, TRPC1, TRPC3 or TRPC6 against the house keeping gene (Actb) calculated in each sample. In SCI mice, TRPV1 mRNA levels in the L6 DRG (n=20 samples from 7 mice) were higher than in spinal intact (SI) mice (n=15 samples from 8 mice), and TRPC3 and TRPC6 mRNA levels in the L6 DRG were lower than in spinal intact mice. After anti-NGF treatment, TRPV1 levels in SCI mice were lower than in SCI mice without treatment (n=20 samples from 10 mice), and TRPC3 and TRPC6 were higher than in SCI mice without treatment. In addition, there were no significant differences in TRPV1, TRPC1, TRPC3 or TRPC6 expression between SI mice and SCI mice with anti NGF treatment.
*P < 0.05 versus spinal intact (SI) mice, †P < 0.05 versus SCI mice without treatment.