Figure 2.
Effect of different regulatory complexes on ATPase activities in the absence of Ca2+. Three TnC mutants, one TnT mutant (Δ14), and one double mutant are compared with the wild type (WT), bare actin filaments (actin), and actin-tropomyosin (actin-Tm). The conditions were the same as those described for Figure 1, except that the troponin concentration was fixed at 2.8 μM. Data are plotted with the standard deviation. Of the TnC mutants, only the C84Y mutant was significantly different from the wild type with a p value of 0.008.