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. Author manuscript; available in PMC: 2019 Apr 4.
Published in final edited form as: Biochemistry. 2017 May 31;56(23):2928–2937. doi: 10.1021/acs.biochem.6b01092

Figure 4.

Figure 4.

Effect of different regulatory complexes on ATPase activities at a saturating Ca2+ concentration in the presence of NEM-S1. Three TnC mutants, one TnT mutant (Δ14), and one double mutant are compared with the wild type (WT) and bare actin filaments (actin). The actin data are the same as those from Figure 2 and are included for comparison. The conditions were the same as those described for Figure 1, except that the EGTA was replaced with 0.1 mM CaCl2. NEM-S1 (10 μM) was included in each assay to fully stabilize the active state. Furthermore, the concentrations of actin, tropomyosin, and troponin were increased to 20, 4.4, and 5.7 μM, respectively, to maintain 10 μM free actin as in Figures 1–3. None of the TnC mutants had activities significantly different from that of the wild type (p > 0.33). Data are plotted with the standard deviation.