Figure 1.

The schematic diagram of the proteome microarray strategy for identification of Mtb-human PPI. Briefly, the Mtb secreted protein (SP) and cellular protein (CP) were prepared and labeled with distinct fluorescein, respectively. After incubated with human proteome microarray, the potential Mtb interactors were screened. To call for Mtb effectors, human proteins were expressed and purified in vitro, reversely incubated with Mtb proteome microarray. Following experimental validation by His pull-down assay and Co-IP determined the interactions and the Mtb-host PPI.