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. 2019 Mar 28;9:65. doi: 10.3389/fcimb.2019.00065

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Copyright © 2019 Cao, Lyu, Jia, Wang, Du, Pan, Li, Xing, Xiao, Ma and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of Mtb SPI and CPI on a human proteome microarray. (A) Extraction and purification of Mtb SP and CP. The proteins were detected by SDS-PAGE followed by coomassie brilliant blue staining. (B) The Venn diagram of Mtb SPI (green) and CPI (red). (C) Representatives of the newly identified Mtb SPI and CPI. The cutoff for calling positive candidates was set as signal-to-noise (SNR) ratio > 2.0. Experiments were performed in two replicates.