Figure 2.

Overview of the iterative phage library screening assay in CF mucus model. (A) Mucus containing 2 × 10¹⁰ phage plaque forming units is incubated in the donating reservoir. Phage that penetrate through mucus are collected, quantified by titering, and amplified by E. coli for the next round of screening. (B) Top-down view and side view of a transwell insert containing a mucus layer for screens against CF mucus. (C) Enrichment of phage plaque forming units recovered from the basolateral side after four rounds of screening (R1 to R4, n=1). Phage concentration was quantified by standard plaque assay.