Table 2.
Diffusion coefficients of phage clones in PBS and CF mucus (CM), and ratios of the average apparent diffusion coefficients in PBS (DPBS) compared to CF mucus (DCM). DH, apparent hydrodynamic diameter, D, apparent diffusion coefficient, PBS, phosphate buffered saline, CM, CF mucus.
Phage clone | DH, nma | D, 10−9 cm2 s−1 | DPBS/DCMc | |
---|---|---|---|---|
PBSb | CM | |||
M13KE | 95.09 ± 4.72 | 51.9 ± 2.6 | 0.18 ± 0.03 | 288 |
SSQ | 98.20 ± 2.87 | 50.2 ± 1.5 | 0.20 ± 0.11 | 251 |
ISL | 90.26 ± 2.38 | 54.6 ± 1.4 | 0.47 ± 0.16 | 116 |
YNS | 112.03 ± 1.02 | 43.9 ± 0.4 | 0.17 ± 0.04 | 258 |
Determined by dynamic light scattering.
DPBS is calculated from the Stokes-Einstein equation.
The DPBS/DCM ratio indicates by what multiple the average diffusion coefficient in CF mucus is slower than in saline.